Dose-time and Source-Dependent Analysis of Intraoperative Radiotherapy-Treated Seroma on Breast Cancer Cell Types: A Pilot Study

Background: Intraoperative radiation therapy (IORT) is a novel approach to breast cancer (BC) treatment. Objectives: In this study, we compared the cellular and molecular effects of IORT-treated post-lumpectomy wound fluid (seroma) at point IOeRT versus IOxRT on BC cell line. Methods: Immortalized human lines: MCF-7, MDA-MB-231, MCF10 were incubated with seroma from 3 groups patients (as pilot study). The first group received electron (IOeRT, Boost dose=12Gy), second one (Radical dose=21Gy), third was prescribed x-ray (IOxRT, X-ray=20Gy). Cellular tests used investigate how cells are influenced by seroma. Results: We evaluated dose-time source-dependent lines. observed that IOxRT-treated has most significant reduction proliferation, induced cycle arrest, apoptosis. Furthermore, inhibited migration invasion lines -treated Conclusions: Although suggest 24 h, IORT (specifically IOxRT)-treated may play an important protective role in tumor bed, which followed local recurrence decreases.